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Plant biotechnology questions.

Plant biotechnology questions.

Find Plant biotechnology university examination questions in acaproso.com

# Question
1

Describe the tissue culture laboratory giving examples of tools and equipments you expect to be available there.


Practical
2

A detergent and Sodium hypochlorite are used during preparation of tissue for initiation of culture before transplanting. What are the concentrations and standard times used for sodium hypochlorite? What is the use  of both chemicals in this particular procedure?


Practical
3

Rinsing is done after NaOCl procedure during tissue preparation. How many washes are done with standard procedure and why?


Practical
4

A protocol gives a concentration of 3.283 g/100ml micronutrients required to make a stock solution which is 1000 times stronger as compared to desired concentration of the medium. You want to make up a litre of the medium for the experiment. How much stock will you add to the medium?


Practical
5

What are the following terms in genetic transformation?

  1. npt11
  2. Bar
  3. hpt
  4. BT gene
  5. GUS

 


Short answers
6

Match an item in column A to a corresponding item in column B.

Column A Column B

 

  1. Genetic modification
  2. Nopaline
  3. ORF
  4. Expression cassette
  5. Reporter gene
  6. Marker
  7. Vir gene
  8. Beta gal
  9. SSBP
  10. Anneal
  11. Closing of the full plasmid
  12. Initiator codon for protein synthesis
  13. Terminator codon for protein synthesis
  14. Transcription start site
  15. The first 1,000 bp of the 5` flanking region
  16. Coding section
  17. Non-coding section
  18. Constitutive promoters
  19. Inducible promoters
  20. Phytoene desaturase

 

  1. Stabilizes T DNA template
  2. Lycopene
  3. Mediates transfer of T DNA to host celss
  4. Exon
  5. DNA ligase
  6. Recombine DNA in the Double stranded
  7. Form
  8. IAIA
  9. Binary vector system
  10. Intron
  11. AUG
  12. Expresses in all tissues at all times
  13. Transgenic cells
  14. Indicates presence of promoters
  15. Amino acid
  16. Reports on presence of gene of interest
  17. Mediates transfer of T-DNA
  18. UAG
  19. Gene promoter
  20. Gene construct
  21. Light, heat, wound, or chemical inducibles

 


Matching items
7

List 5 important requirements of the culture room in a basic tissue culture laboratory.


Short answers
8

Tissue culture can produce excessive  amount of volatile compounds such as ethanol, ethylene, acetaldehyde and C_{2}O. Mention the 5 methods of air exchange for minimizing the detrimental effects of the volatile compounds in culture.


Short answers
9

Anther culture has several advantages in cultivar development. What are 4 major problems associated with anther culture?


Short answers
10

Mention any 6 requirements in protoplast isolation medium for protoplast culture.


Short answers