Fundamentals of microbiology UE Past Papers Questions.

Suppose you have a microoganism “X” that you would like to observe. Under what conditions you will use the following microscopes.

1. Dark field microscope
2. Electron microscope
3. Atomic force microscope

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Calculate the dilution factor given the original spore concentration of Asperigillus niger is spore/ml. The inoculum concentration required to inoculate test plants is spores/ml.

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You have been provided with light microscope which has the following lenses. Eye pieces lens x10 objective lenses x5 x10 x40 x100

1. If someone uses x40, what is the total magnification of the object
2. What is the maximum magnification of the microscope?

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Outline four ways of preventing contamination to maintain sterility.

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Media selection to suit growth of a particular microbe is an essential consideration. At what time you will use the following media.

1. Selective media
2. Differential media
3. Enrichment media
4. Defined media

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