Plant biotechnology UE Past Papers Questions.

Describe the tissue culture laboratory giving examples of tools and equipments you expect to be available there.

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A detergent and Sodium hypochlorite are used during preparation of tissue for initiation of culture before transplanting. What are the concentrations and standard times used for sodium hypochlorite? What is the use  of both chemicals in this particular procedure?

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Rinsing is done after procedure during tissue preparation. How many washes are done with standard procedure and why?

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A protocol gives a concentration of 3.283 g/100ml micronutrients required to make a stock solution which is 1000 times stronger as compared to desired concentration of the medium. You want to make up a litre of the medium for the experiment. How much stock will you add to the medium?

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What are the following terms in genetic transformation?

1. npt11
2. Bar
3. hpt
4. BT gene
5. GUS

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